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ac recombinant human vegf protein r d systems  (R&D Systems)


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    R&D Systems ac recombinant human vegf protein r d systems
    Ac Recombinant Human Vegf Protein R D Systems, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 607 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rat+recombinant+vegf/Recombinant+Human%2FMouse%2FRat+Activin+A+Protein/pm40779394-193-279-284
    Average 96 stars, based on 607 article reviews
    ac recombinant human vegf protein r d systems - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Recombinant:

    Article Title: The influence of vascularization of transplanted processed allograft nerve on return of motor function in rats.
    Article Snippet: Processed nerve allografts have become an alternative to repair segmental nerve defects, with results comparable with autografts regarding sensory recovery; however, they have failed to reproduce comparable motor recovery.. The purpose of this study was to determine how revascularizaton of processed nerve allograft would affect motor recovery.. Eighty-eight rats were divided in four groups of 22 animals each.

    Article Title: Proteolytic cleavage of vascular adhesion protein-1 induced by vascular endothelial growth factor in retinal capillary endothelial cells.
    Article Snippet: .. The TR-iBRB2 cells were washed twice with serum-free DMEM and stimulated with rat recombinant VEGF (10 to 100ng/ml, R&D systems) in serum-free DMEM for 17 h and the supernatants were harvested. .. Since spermine is a substrate for both SSAO and spermine oxidase (SMOX), the supernatants were pretreated with a SMOX inhibitor, MDL72527 [22] (50μM, Sigma) at 37°C for 30 min and then incubated with 200μM spermine (Sigma) at 37°C for 2 h. Hydrogen peroxide concentration in the supernatant was measured using a Fluoro hydrogen peroxide kit (Cell technology).

    Article Title: Proteolytic cleavage of vascular adhesion protein-1 induced by vascular endothelial growth factor in retinal capillary endothelial cells.
    Article Snippet: .. The TR-iBRB2 cells were stimulated with rat recombinant VEGF (100ng/ml, R&D systems) for 17 h. The supernatants were harvested and concentrated through centrifugal ultrafiltration (Amicon ultra 3kDa), and gelatin zymoglaphy was performed according to the manufacture’s protocol (Cosmobio, Tokyo, Japan). .. The density of negatively stained bands was measured with ImageJ software (available from http s:// imag ej.nih.gov/ij/; developed by Wayne Rasband, National Institutes of Health, Bethesda, MD, USA).

    Article Title: Proteolytic cleavage of vascular adhesion protein-1 induced by vascular endothelial growth factor in retinal capillary endothelial cells.
    Article Snippet: Since spermine is a substrate for both SSAO and spermine oxidase (SMOX), the supernatants were pretreated with a SMOX inhibitor, MDL72527 [22] (50μM, Sigma) at 37°C for 30 min and then incubated with 200μM spermine (Sigma) at 37°C for 2 h. Hydrogen peroxide concentration in the supernatant was measured using a Fluoro hydrogen peroxide kit (Cell technology). .. Reactive oxygen species (ROS) detection assay TR-iBRB2 cells were washed twice with serum-free DMEM and stimulated with rat recombinant VEGF (100ng/ml, R&D systems) and VAP-1 inhibitor semicarbazide (100μM, Cell technology) for 17 h. The supernatants were collected and mixed with 50μM MDL72527 at 37°C for 30 min, and then incubated with 200μM spermine at 37°C for 24 h. The solution was added to freshly-seeded TR-iBRB2 cells and incubated for 24 h. Thereafter, ROS activity was measured using DCFDA-Cellular ROS Detection Assay Kit (Abcam), following the manufacturer’s protocol. ..

    Article Title: Effect of Vascular Endothelial Growth Factor Administration on Nerve Regeneration after Autologous Nerve Grafting.
    Article Snippet: Reconstruction of segmental defects of peripheral nerves remains a challenging clinical problem.. Although autologous nerve graft has been widely accepted as the gold standard treatment, the results are still far from satisfactory.. The literature shows that less than 25% of patients who received an autologous nerve graft of the median nerve at the wrist level, regain full motor function and only 1 to 3% recovered normal sensation after 5 years.1,2 Bioabsorbable nerve

    Article Title: Norrin restores blood-retinal barrier properties after vascular endothelial growth factor–induced permeability
    Article Snippet: .. Male Long-Evans rats (Charles River Laboratories, Wilmington, MA) weighting 200-250g were anesthetized with ketamine/xylazine (66.7 and 6.7 mg/kg of body weight, respectively), to receive an intravitreal (IVT) injection of vehicle (0.1% BSA/PBS + 4 mM HCl), 50 ng of rat recombinant VEGF (diluted in 0.1% BSA/PBS) (R&D systems, Minneapolis, MN; 564-RV) and/or 40 ng of recombinant human norrin (diluted in 4 mM HCl) (R&D systems; 3014-NR) per eye. ..

    Article Title: Proteolytic cleavage of vascular adhesion protein-1 induced by vascular endothelial growth factor in retinal capillary endothelial cells.
    Article Snippet: .. TR-iBRB2 cells were stimulated with rat recombinant VEGF (100ng/ml, R&D systems) for 17 h with or without broad spectrum MMP inhibitor Batimastat (Tocris Bioscience, Bristol, UK). ..

    Article Title: Proteolytic cleavage of vascular adhesion protein-1 induced by vascular endothelial growth factor in retinal capillary endothelial cells.
    Article Snippet: Signals were visualized with chemiluminescence (SuperSignal West Pico; Thermo Fisher scientific, Waltham, MA,USA), according to the manufacturer’s protocol. .. The TR-iBRB2 cells were washed twice with serum-free DMEM and stimulated with rat recombinant VEGF (1 to 100ng/ml, R&D systems) in serum-free DMEM for 17 h and the supernatants were harvested and centrifuged at 500g for 10 min at 4°C. ..

    Saline:

    Article Title: The influence of vascularization of transplanted processed allograft nerve on return of motor function in rats.
    Article Snippet: Processed nerve allografts have become an alternative to repair segmental nerve defects, with results comparable with autografts regarding sensory recovery; however, they have failed to reproduce comparable motor recovery.. The purpose of this study was to determine how revascularizaton of processed nerve allograft would affect motor recovery.. Eighty-eight rats were divided in four groups of 22 animals each.

    Article Title: Effect of Vascular Endothelial Growth Factor Administration on Nerve Regeneration after Autologous Nerve Grafting.
    Article Snippet: Reconstruction of segmental defects of peripheral nerves remains a challenging clinical problem.. Although autologous nerve graft has been widely accepted as the gold standard treatment, the results are still far from satisfactory.. The literature shows that less than 25% of patients who received an autologous nerve graft of the median nerve at the wrist level, regain full motor function and only 1 to 3% recovered normal sensation after 5 years.1,2 Bioabsorbable nerve

    Detection Assay:

    Article Title: Proteolytic cleavage of vascular adhesion protein-1 induced by vascular endothelial growth factor in retinal capillary endothelial cells.
    Article Snippet: Since spermine is a substrate for both SSAO and spermine oxidase (SMOX), the supernatants were pretreated with a SMOX inhibitor, MDL72527 [22] (50μM, Sigma) at 37°C for 30 min and then incubated with 200μM spermine (Sigma) at 37°C for 2 h. Hydrogen peroxide concentration in the supernatant was measured using a Fluoro hydrogen peroxide kit (Cell technology). .. Reactive oxygen species (ROS) detection assay TR-iBRB2 cells were washed twice with serum-free DMEM and stimulated with rat recombinant VEGF (100ng/ml, R&D systems) and VAP-1 inhibitor semicarbazide (100μM, Cell technology) for 17 h. The supernatants were collected and mixed with 50μM MDL72527 at 37°C for 30 min, and then incubated with 200μM spermine at 37°C for 24 h. The solution was added to freshly-seeded TR-iBRB2 cells and incubated for 24 h. Thereafter, ROS activity was measured using DCFDA-Cellular ROS Detection Assay Kit (Abcam), following the manufacturer’s protocol. ..

    Incubation:

    Article Title: Proteolytic cleavage of vascular adhesion protein-1 induced by vascular endothelial growth factor in retinal capillary endothelial cells.
    Article Snippet: Since spermine is a substrate for both SSAO and spermine oxidase (SMOX), the supernatants were pretreated with a SMOX inhibitor, MDL72527 [22] (50μM, Sigma) at 37°C for 30 min and then incubated with 200μM spermine (Sigma) at 37°C for 2 h. Hydrogen peroxide concentration in the supernatant was measured using a Fluoro hydrogen peroxide kit (Cell technology). .. Reactive oxygen species (ROS) detection assay TR-iBRB2 cells were washed twice with serum-free DMEM and stimulated with rat recombinant VEGF (100ng/ml, R&D systems) and VAP-1 inhibitor semicarbazide (100μM, Cell technology) for 17 h. The supernatants were collected and mixed with 50μM MDL72527 at 37°C for 30 min, and then incubated with 200μM spermine at 37°C for 24 h. The solution was added to freshly-seeded TR-iBRB2 cells and incubated for 24 h. Thereafter, ROS activity was measured using DCFDA-Cellular ROS Detection Assay Kit (Abcam), following the manufacturer’s protocol. ..

    Activity Assay:

    Article Title: Proteolytic cleavage of vascular adhesion protein-1 induced by vascular endothelial growth factor in retinal capillary endothelial cells.
    Article Snippet: Since spermine is a substrate for both SSAO and spermine oxidase (SMOX), the supernatants were pretreated with a SMOX inhibitor, MDL72527 [22] (50μM, Sigma) at 37°C for 30 min and then incubated with 200μM spermine (Sigma) at 37°C for 2 h. Hydrogen peroxide concentration in the supernatant was measured using a Fluoro hydrogen peroxide kit (Cell technology). .. Reactive oxygen species (ROS) detection assay TR-iBRB2 cells were washed twice with serum-free DMEM and stimulated with rat recombinant VEGF (100ng/ml, R&D systems) and VAP-1 inhibitor semicarbazide (100μM, Cell technology) for 17 h. The supernatants were collected and mixed with 50μM MDL72527 at 37°C for 30 min, and then incubated with 200μM spermine at 37°C for 24 h. The solution was added to freshly-seeded TR-iBRB2 cells and incubated for 24 h. Thereafter, ROS activity was measured using DCFDA-Cellular ROS Detection Assay Kit (Abcam), following the manufacturer’s protocol. ..

    Injection:

    Article Title: Norrin restores blood-retinal barrier properties after vascular endothelial growth factor–induced permeability
    Article Snippet: .. Male Long-Evans rats (Charles River Laboratories, Wilmington, MA) weighting 200-250g were anesthetized with ketamine/xylazine (66.7 and 6.7 mg/kg of body weight, respectively), to receive an intravitreal (IVT) injection of vehicle (0.1% BSA/PBS + 4 mM HCl), 50 ng of rat recombinant VEGF (diluted in 0.1% BSA/PBS) (R&D systems, Minneapolis, MN; 564-RV) and/or 40 ng of recombinant human norrin (diluted in 4 mM HCl) (R&D systems; 3014-NR) per eye. ..



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    Image Search Results


    VEGF-C 152s treatment prior to ovariectomy limited the negative effects of subsequent E2 supplementation on lymphatic function and plaque burden in ldlr −/− mice. (A) Experimental design. 7 weeks old Ldlr −/− female mice received IP injections of VEGF-C 152s (e.g., VEGF-C) or vehicle. At 11 weeks, mice underwent either bilateral ovariectomy (OVX) or sham surgery, and switched on a high-fat diet (HFD). Estrogen (E2) supplementation began one week later. Lymphatic function was assessed in mice immediately prior to sacrifice at 20.5 weeks, after which organs were harvested. Green, orange and pink dashed lines represent VEGF-C 152s injections, HFD, and E2 implant durations, respectively. (B) Total body weight at the time of sacrifice and (C) over 21 weeks were reported. (D) Weight of visceral adipose tissue and (E) weight of the uterus, each expressed as percentage of total body weight. (F) Plasma total cholesterol and, (G) lipoprotein profile were assessed. (H) Contraction frequency of the popliteal collecting lymphatic vessels was measured by intravital microscopy. (I) Representative images of en face oil-red-O staining of the aorta and quantification of atherosclerotic plaque burden, expressed as percentage of total aortic area. Data points represent individual animals. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. HFD, high-fat diet; OVX, ovariectomy; VEGF-C, vascular endothelial growth factor C; CM, chylomicron; VLDL, very-low density lipoprotein; LDL, low-density lipoprotein; HDL, high-density lipoprotein.

    Journal: Frontiers in Cardiovascular Medicine

    Article Title: Sex-specific lymphatic responses to estrogen shape atherosclerosis in high-risk mice

    doi: 10.3389/fcvm.2026.1699372

    Figure Lengend Snippet: VEGF-C 152s treatment prior to ovariectomy limited the negative effects of subsequent E2 supplementation on lymphatic function and plaque burden in ldlr −/− mice. (A) Experimental design. 7 weeks old Ldlr −/− female mice received IP injections of VEGF-C 152s (e.g., VEGF-C) or vehicle. At 11 weeks, mice underwent either bilateral ovariectomy (OVX) or sham surgery, and switched on a high-fat diet (HFD). Estrogen (E2) supplementation began one week later. Lymphatic function was assessed in mice immediately prior to sacrifice at 20.5 weeks, after which organs were harvested. Green, orange and pink dashed lines represent VEGF-C 152s injections, HFD, and E2 implant durations, respectively. (B) Total body weight at the time of sacrifice and (C) over 21 weeks were reported. (D) Weight of visceral adipose tissue and (E) weight of the uterus, each expressed as percentage of total body weight. (F) Plasma total cholesterol and, (G) lipoprotein profile were assessed. (H) Contraction frequency of the popliteal collecting lymphatic vessels was measured by intravital microscopy. (I) Representative images of en face oil-red-O staining of the aorta and quantification of atherosclerotic plaque burden, expressed as percentage of total aortic area. Data points represent individual animals. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. HFD, high-fat diet; OVX, ovariectomy; VEGF-C, vascular endothelial growth factor C; CM, chylomicron; VLDL, very-low density lipoprotein; LDL, low-density lipoprotein; HDL, high-density lipoprotein.

    Article Snippet: Mice received intraperitoneal (IP) injections of VEGF-C (152s) at a dose of 50 ng per 25 g of body weight [purified recombinant rat VEGF-C protein (152s), Fitzgerald, catalog no. 30R-AV006-22] or vehicle control (phosphate-buffered saline, PBS), administered three times per week for four consecutive weeks.

    Techniques: Clinical Proteomics, Intravital Microscopy, Staining

    Figure 6. Cultured retinal progenitor cell groups with Top2b overexpression (OE) and knockdown (KD) illustrate altered expression of select chemotactic receptors compared to wildtype cell groups with no Top2b manipulation (WT). Cell groups illustrated significant differences in the expression of selected chemotactic receptors: vascular endothelial growth factor receptor (VEGFR1), fibroblast growth factor receptor (FGFR1), and c-x-c chemokine receptor 4 (CXCR4, receptor for the stromal cell-derived factor 1, SDF-1a, ligand). Statistical significance across the cell groups is denoted by p-value: * < 0.05, ** < 0.01, **** < 0.0001 as via two-way ANOVA tests followed by Dunnett’s post-hoc test

    Journal: Exploration of BioMat-X

    Article Title: Top2b regulates morphological and migratory properties of retinal progenitor cells in vivo and upon transplantable matrix substrates

    doi: 10.37349/ebmx.2025.101335

    Figure Lengend Snippet: Figure 6. Cultured retinal progenitor cell groups with Top2b overexpression (OE) and knockdown (KD) illustrate altered expression of select chemotactic receptors compared to wildtype cell groups with no Top2b manipulation (WT). Cell groups illustrated significant differences in the expression of selected chemotactic receptors: vascular endothelial growth factor receptor (VEGFR1), fibroblast growth factor receptor (FGFR1), and c-x-c chemokine receptor 4 (CXCR4, receptor for the stromal cell-derived factor 1, SDF-1a, ligand). Statistical significance across the cell groups is denoted by p-value: * < 0.05, ** < 0.01, **** < 0.0001 as via two-way ANOVA tests followed by Dunnett’s post-hoc test

    Article Snippet: Approximately 600 μL of each growth factor vascular endothelial growth factor (VEGF; R&D Systems, Minneapolis, MN, USA, 564-RV), fibroblast growth factor-8 (FGF-8; Invitrogen, Carlsbad, CA, USA, PHG0184), and stromal derived factor 1-a (SDF-1a; Sigma Aldrich, St. Louis, MO, USA, SRP3276) was added to the bottom of separate transwell assays.

    Techniques: Cell Culture, Over Expression, Knockdown, Expressing, Derivative Assay

    Effects of ROX and/or sh-Vegfr2 on VEGFR2 protein expression in rat vascular endothelial cells. ( A ) Western blot bands of indicating proteins in endothelial cells; ( B ) densitometric analysis of the ratio of the intensity of protein bands. Values are the mean ± SD of VEGFR2 expression standardized to β-actin expression in three independent experiments; ** p < 0.01, compared with the PBS group; ## p < 0.01, compared with the ROX group.

    Journal: Veterinary Sciences

    Article Title: A Recombinant Lentiviral Vegfr2-Silencing Vector Attenuates Roxarsone-Promoted Growth of Rat Vascular Endothelial Cells and Angiogenesis in Matrigel Plug and B16F10 Xenograft Models

    doi: 10.3390/vetsci11100451

    Figure Lengend Snippet: Effects of ROX and/or sh-Vegfr2 on VEGFR2 protein expression in rat vascular endothelial cells. ( A ) Western blot bands of indicating proteins in endothelial cells; ( B ) densitometric analysis of the ratio of the intensity of protein bands. Values are the mean ± SD of VEGFR2 expression standardized to β-actin expression in three independent experiments; ** p < 0.01, compared with the PBS group; ## p < 0.01, compared with the ROX group.

    Article Snippet: Rabbit anti-rat VEGFR2 polyclonal antibody, mouse anti-rat β-actin monoclonal antibody, HRP-labeled goat anti-rabbit IgG, HRP-labeled goat anti-mouse IgG and SABC-POD three-step detection kit were purchased from Boster Biological Technology Co., Ltd. (Wuhan, China).

    Techniques: Expressing, Western Blot

    Effects of ROX and/or sh-Vegfr2 on proliferation and migration of endothelial cells. ( A ) BrdU-positive cell rate; ( B ) migration distance statistics chart. Data expressed as mean ± SD; * p < 0.05, ** p < 0.01, compared with the PBS group; ## p < 0.01, compared with the ROX group.

    Journal: Veterinary Sciences

    Article Title: A Recombinant Lentiviral Vegfr2-Silencing Vector Attenuates Roxarsone-Promoted Growth of Rat Vascular Endothelial Cells and Angiogenesis in Matrigel Plug and B16F10 Xenograft Models

    doi: 10.3390/vetsci11100451

    Figure Lengend Snippet: Effects of ROX and/or sh-Vegfr2 on proliferation and migration of endothelial cells. ( A ) BrdU-positive cell rate; ( B ) migration distance statistics chart. Data expressed as mean ± SD; * p < 0.05, ** p < 0.01, compared with the PBS group; ## p < 0.01, compared with the ROX group.

    Article Snippet: Rabbit anti-rat VEGFR2 polyclonal antibody, mouse anti-rat β-actin monoclonal antibody, HRP-labeled goat anti-rabbit IgG, HRP-labeled goat anti-mouse IgG and SABC-POD three-step detection kit were purchased from Boster Biological Technology Co., Ltd. (Wuhan, China).

    Techniques: Migration

    Effects of ROX and/or sh-Vegfr2 on tube formation of endothelial cells. ( A ) Number of nodes forming a tube-like structure; ( B ) branch lengths forming the tube-like structure. Data expressed as mean ± SD; ** p < 0.01, compared with the PBS group; ## p < 0.01, compared with the ROX group.

    Journal: Veterinary Sciences

    Article Title: A Recombinant Lentiviral Vegfr2-Silencing Vector Attenuates Roxarsone-Promoted Growth of Rat Vascular Endothelial Cells and Angiogenesis in Matrigel Plug and B16F10 Xenograft Models

    doi: 10.3390/vetsci11100451

    Figure Lengend Snippet: Effects of ROX and/or sh-Vegfr2 on tube formation of endothelial cells. ( A ) Number of nodes forming a tube-like structure; ( B ) branch lengths forming the tube-like structure. Data expressed as mean ± SD; ** p < 0.01, compared with the PBS group; ## p < 0.01, compared with the ROX group.

    Article Snippet: Rabbit anti-rat VEGFR2 polyclonal antibody, mouse anti-rat β-actin monoclonal antibody, HRP-labeled goat anti-rabbit IgG, HRP-labeled goat anti-mouse IgG and SABC-POD three-step detection kit were purchased from Boster Biological Technology Co., Ltd. (Wuhan, China).

    Techniques:

    Effects of ROX and/or sh-Vegfr2 on the mouse matrigel plug model. ( A ) Pictures of the mouse matrigel plugs (a: PBS group, b: NC group, c: ROX group, d: sh-Vegfr2 group, and e: sh-Vegfr2 + ROX group); ( B ) hemoglobin content in matrigel plugs. Data expressed as mean ± SD; * p < 0.05, ** p < 0.01, compared with the PBS group; # p < 0.05, compared with the ROX group.

    Journal: Veterinary Sciences

    Article Title: A Recombinant Lentiviral Vegfr2-Silencing Vector Attenuates Roxarsone-Promoted Growth of Rat Vascular Endothelial Cells and Angiogenesis in Matrigel Plug and B16F10 Xenograft Models

    doi: 10.3390/vetsci11100451

    Figure Lengend Snippet: Effects of ROX and/or sh-Vegfr2 on the mouse matrigel plug model. ( A ) Pictures of the mouse matrigel plugs (a: PBS group, b: NC group, c: ROX group, d: sh-Vegfr2 group, and e: sh-Vegfr2 + ROX group); ( B ) hemoglobin content in matrigel plugs. Data expressed as mean ± SD; * p < 0.05, ** p < 0.01, compared with the PBS group; # p < 0.05, compared with the ROX group.

    Article Snippet: Rabbit anti-rat VEGFR2 polyclonal antibody, mouse anti-rat β-actin monoclonal antibody, HRP-labeled goat anti-rabbit IgG, HRP-labeled goat anti-mouse IgG and SABC-POD three-step detection kit were purchased from Boster Biological Technology Co., Ltd. (Wuhan, China).

    Techniques:

    Effects of ROX and/or sh-Vegfr2 on the growth and angiogenesis of/in melanoma xenografts. ( A ) Melanoma xenograft tumor volumes during the test period; ( B ) melanoma xenograft tumor weight at necropsy; ( C ) immunohistochemical expression of CD34 in melanoma xenografts. Data expressed as mean ± SD; * p < 0.05, compared with the PBS group; ## p < 0.01, compared with the ROX group.

    Journal: Veterinary Sciences

    Article Title: A Recombinant Lentiviral Vegfr2-Silencing Vector Attenuates Roxarsone-Promoted Growth of Rat Vascular Endothelial Cells and Angiogenesis in Matrigel Plug and B16F10 Xenograft Models

    doi: 10.3390/vetsci11100451

    Figure Lengend Snippet: Effects of ROX and/or sh-Vegfr2 on the growth and angiogenesis of/in melanoma xenografts. ( A ) Melanoma xenograft tumor volumes during the test period; ( B ) melanoma xenograft tumor weight at necropsy; ( C ) immunohistochemical expression of CD34 in melanoma xenografts. Data expressed as mean ± SD; * p < 0.05, compared with the PBS group; ## p < 0.01, compared with the ROX group.

    Article Snippet: Rabbit anti-rat VEGFR2 polyclonal antibody, mouse anti-rat β-actin monoclonal antibody, HRP-labeled goat anti-rabbit IgG, HRP-labeled goat anti-mouse IgG and SABC-POD three-step detection kit were purchased from Boster Biological Technology Co., Ltd. (Wuhan, China).

    Techniques: Immunohistochemical staining, Expressing